Viruses are a serious threat to human health, and the infectious diseases caused by them pose a major threat to human health, social economy and national security. A comprehensive and in-depth analysis of the infection process of viruses is essential for understanding their pathogenic mechanisms, establishing treatment methods, and formulating prevention and control measures. However, virus infections are extremely complex and dynamic processes, and there is an urgent need to establish methods and technologies for the accurate acquisition of their biochemical information.
Our group has long focused on the key scientific need of dynamic visualization and quantitative analysis of viral infection process. Combined with multidisciplinary research methods and technologies such as biomaterials, bioimaging, and chemical biology, We constructed a series of biological probes to track different components of individual viruses, developed a series of real-time, in situ, quantitative dynamic visualization methods to detect individual viral infection process, and achieved the goal of realizing the dynamic visualization of virus-host interaction in living cells. We have developed a series of dynamic visualization methods for real-time, in situ and quantitative detection of individual viral infections, and achieved a series of innovative results in the accurate acquisition of information related to virus-host interactions in living cells and the in-depth study of related viral infection mechanisms.
Viral infection is a dynamic and complex process in which viruses interact dynamically with host cells, including a number of sequential steps such as virus-specific adsorption, entry, intracellular translocation, decapsidation, nucleic acid transcription, viral particle assembly and release, each of which involves a variety of pathways and mechanisms. SVT is often based on fluorescence imaging and allows one to follow individual viruses through different parts of their life cycle, thus providing a dynamic understanding of the underlying processes of viruses that occur in living cells.
The lipids of cell membranes play an important role in the release of viral nucleic acids as the primary barrier to virus-host interactions. We have established a highly sensitive in situ, real-time, quantitative method for the detection of lipid molecules in living cell membranes, capable of accurately obtaining the content of lipid molecules on the inner and outer leaflets of the cytoplasmic membrane, and have explored the dynamic molecular mechanisms of viral invasion and lipid interactions. Further based on the properties of viruses and lipid molecules themselves, we developed virus-mimicking lipid nanoparticles as a multifunctional nanoplatform for in vivo imaging and therapy.